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Post larvae analysis - Microscopic

MGR (Muscle Gut Ratio)

The ventral muscle in the 6th abdominal segment under the intestine is one of the largest in the post larvae shrimp. The ratio between the width of this muscles and the width of the gut has already been proposed and used in PL quality control (Muscle Gut Ratio of 4:1 based on comparisons with wild post larvae). However, in practice, it was found that this measurement can be difficult due to the movement of the shrimp on the slide. Killing the PL also led to post mortem changes which did not reflect the true condition of the muscle. To overcome this, we use a 50% criterion. If the muscle constitutes less then 50% of the width of the tail at the midpoint of the 6th abdominal segment the quality is unacceptable. More than 50% is acceptable.

Good muscle condition. The tail muscle cover more than 50% of the width of the tail (6th segment).
Poor quality tail muscle. The muscle in stretched and covers less than 50% of the width of the tail (6th abdominal) segment. Note also the expanded intestine.
Poor muscle condition. The tail muscle is discolored and thin. Note also the nematocysts clumping on the out side of the intestine. This indicates an infection.

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